Factors affecting transformation of Micrococcus lysodeikticus.
نویسنده
چکیده
Relatively high transfo mation frequencies were obtained with Micrococcus lysodeikticus cells, previou[ill]sly grown in defined broth, with a tris(hydroxymethyl)-aminomethane-glutamate b uffer containing Mg(++), Ca(++), Ba(++), or Sr(++).
منابع مشابه
Nonphotoreactivating Repair of Ultraviolet Light-Damaged Transforming Deoxyribonucleic Acid by Micrococcus lysodeikticus Extracts.
Elder, Robert L. (Johns Hopkins University, Baltimore, Md.), and Roland F. Beers, Jr. Nonphotoreactivating repair of ultraviolet light-damaged transforming deoxyribonucleic acid by Micrococcus lysodeikticus extracts. J. Bacteriol. 90:681-686. 1965.-Extracts from Micrococcus lysodeikticus repair Haemophilus influenzae transforming deoxyribonucleic acid (DNA) damaged by ultraviolet light radiatio...
متن کاملInduction of lysogenic Micrococcus lysodeikticus.
Field, A. K. (Cornell University, Ithaca, N.Y.) and H. B. Naylor. Induction of lysogenic Micrococcus lysodeikticus. J. Bacteriol. 84:1129-1133. 1962.-Between 50 and 60% of log-phase cells of Micrococcus lysodeikticus strain ML 53-40, lysogenic for N5 bacteriophage, were induced by an optimal dose of ultraviolet light. Induction of dilute cell suspensions caused a subsequent 200- to 800-fold inc...
متن کاملCharacterization of purines and pyrimidines of deoxypentose nucleic acids from Micrococcus lysodeikticus and bacteriophage.
The phenomenon of abortive infection in the Micrococcus lysodeikticus-bacteriophage system described by Naylor and Burgi (1956) constituted the basic problem to which the following study was directed. The role of deoxypentose nucleic acids (DNA) in cellular physiology and the role of certain of them in transformation experiments is well known. (Avery et al., 1944; Hotchkiss, 1951; Zamenhof et a...
متن کاملNonphotoreactivating Repair of Ultraviolet Light-damaged Micrococcus Lysodeikticus Cells.
Elder, Robert L. (Johns Hopkins University, Baltimore, Md.), and Roland F. Beers, Jr. Nonphotoreactivating repair of ultraviolet light-damaged Micrococcus lysodeikticus cells. J. Bacteriol. 89:1225-1230. 1965.-Micrococcus lysodeikticus cells are highly resistant to ultraviolet radiation. The shape of the dose-survival curve is consistent with the presence of a repair mechanism. The presence of ...
متن کاملNeutralization of human serum lysozyme by sodium polyanethol sulfonate but not by sodium amylosulfate.
Sodium polyanethol sulfonate (SPS) at 500 microgram/ml, but not sodium amylosulfate (SAS) at 500 microgram/ml, precipitated egg white lysozyme (1 mg and 50 microgram of lysozyme per ml) as determined with the assay strain Micrococcus lysodeikticus ATCC 4698. Fresh and heat-inactivated (56 degrees C, 30 min) human serum (80%, vol/vol) killed M. lysodeikticus (10(4) bacteria per ml at zero time) ...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 98 3 شماره
صفحات -
تاریخ انتشار 1969